A Preliminary Study of Bacteriocins Effects from Lactobacillus bulgaricus onTumor Necrosis Factor– α (TNF-α) in Preeclampsia Trophoblast Cell

Abstract

Bacteriocins is metabolite produce from probiotic bacteria i.e Lactobacillus bulgaricus ATCC 11842, which can enhance the immune system (immunomodulator). In vitro laboratory analytic study has been conducted to identify the possibility of bacteriocins as immunomodulator on decreasing TNF-α levels as molecular marker in preeclampsia pregnancy. This study used experimental method with 4 stages; preparation of bacteriocins, identification of probiotic bacteria’s growth and pH curve, normal and preeclampsia trophoblast primary cell culture, and determination of TNF-α levels. The data was analyzed by ANOVA followed by Duncan’s multiple range test (DMRT). Decreased levels of TNF-α were very significant, with p <0.001. In conclusion, bacteriocins has the ability as an immunomodulator because treatment with bacteriocins can decrease the level of TNF-α in preeclampsia trophoblast cell.